Plate-based biochemical assay using quantum dots as a fluorescent labeling agent

Chin Ping Huang, Hong Wei Liu, Chao Yun Tsao, Li Te Yin, Su Feng Chiu, Teng-Ming Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A biochemical assay based on fluorescence imaging analysis by confocal laser scanning as a detection technique is specified. The method involved a glass plate coated with nitrocellulose (NC), where α-human-IgE-biotin at various concentrations was immobilized on an NC plate. Luminescent core-shell CdSe/ZnS QD-SA conjugates were then used as a fluorescent labeling agent to be captured specifically by biotinated α-human-IgE immobilized in a micro-array by affinity binding between SA and biotin in a plate-based biochemical assay. A confocal laser scanner was used to detect the fluorescence signals from the α-human-IgE-biotin-SA-QD complex. Experimental findings reveal that fluorescence intensity of QD-SA saturated at QD concentrations above 0.4 mg/ml. Moreover, a calibration curve between the fluorescence intensity and the concentration of α-human-IgE-biotin is plotted. The range of detectable concentrations of biotinlated α-human IgE was found to be between 3.3 and 100 μg/ml in the α-human-IgE-biotin-SA-QD complex. Therefore, the results were consistent with the binding specificity in a plate-based biochemical assay, as determined using luminescent QDs as a labeling agent.

Original languageEnglish
Pages (from-to)713-720
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume108
Issue number1-2 SPEC. ISS.
DOIs
StatePublished - 22 Jul 2005

Keywords

  • Biochemical assay
  • Fluorescence
  • Quantum dots (QD)
  • Streptavidin (SA)

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    Huang, C. P., Liu, H. W., Tsao, C. Y., Yin, L. T., Chiu, S. F., & Chen, T-M. (2005). Plate-based biochemical assay using quantum dots as a fluorescent labeling agent. Sensors and Actuators, B: Chemical, 108(1-2 SPEC. ISS.), 713-720. https://doi.org/10.1016/j.snb.2004.11.051