Plasmon-induced photoelectrochemical biosensor for in situ real-time measurement of biotin-streptavidin binding kinetics under visible light irradiation

Jingchun Guo, Tomoya Oshikiri, Kosei Ueno, Xu Shi, Hiroaki Misawa

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We developed a localized surface plasmon-induced visible light-responsive photoelectrochemical (PEC) biosensor using a titanium dioxide (TiO2) photoelectrode loaded with gold nanoislands (AuNIs) for in situ real-time measurement of biotin-streptavidin association. As a proof of concept, self-assembled thiol-terminated biotin molecules bound on a AuNls/TiO2 photoelectrode were successfully utilized to explore the photocurrent response to streptavidin-modified gold nanoparticle (STA-AuNP) solutions. This plasmon-induced PEC biosensor is simple and easy to miniaturize. Additionally, the PEC biosensor achieves highly sensitive measurements under only visible light irradiation and prevents the UV-induced damage of samples. Furthermore, a novel approach has been proposed to realize the real-time monitoring of biotin-STA binding affinities and kinetics by analyzing the PEC sensing characteristics. This PEC biosensor and novel analysis method could provide a new approach for the specific electrical detection and real-time kinetic measurements for clinical diagnostics and drug development. (C) 2016 The Authors. Published by Elsevier B.V
Original languageAmerican English
Pages (from-to)70-75
Number of pages6
JournalAnalytica Chimica Acta
Volume957
DOIs
StatePublished - 8 Mar 2017

Keywords

  • Photoelectrochemical biosensor; Localized surface plasmon; Kinetic analysis; Visible light-response
  • GOLD NANOPARTICLES; TIO2 FILMS; RESONANCE; ENHANCEMENT; SPECTROSCOPY; GENERATION; DYNAMICS; SURFACES

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