A nonscanning two-color infrared super-resolution microscope combining a laser fluorescence microscope and picosecond time-resolved transient fluorescence detected IR (TFD-IR) spectroscopy has been developed. Picosecond time-resolved infrared super-resolution images were obtained with 2 ps time resolutions and 1.4 mu m spatial resolutions, without XY scanning of the sample or the excitation light of infrared and visible. A demonstration measurement on Arabidopsis thaliana roots labeled by Rhodamine 6G is presented.
- VIBRATIONAL-ENERGY REDISTRIBUTION
- LARGE POLYATOMIC-MOLECULES
- DETECTED IR SPECTROSCOPY