Photobleaching on photonic crystal enhanced fluorescence surfaces

Vikram Chaudhery, Meng Lu, Cheng-Sheng Huang, Sherine George, Brian T. Cunningham*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The effect of resonant fluorescent enhancement from a photonic crystal surface upon the fluorescent photobleaching rate of Cyanine-5 labeled protein has been investigated. We show that the enhanced excitation mechanism for photonic crystal enhanced fluorescence, in which the device surface resonantly couples light from an excitation laser, accelerates photobleaching in proportion to the coupling efficiency of the laser to the photonic crystal. We also show that the enhanced extraction mechanism, in which the photonic crystal directs emitted photons approximately normal to the surface, does not play a role in the rate of photobleaching. We show that the photobleaching rate of dye molecules on the photonic crystal surface is accelerated by 30x compared to an ordinary glass surface, but substantial signal gain is still evident, even after extended periods of continuous illumination at the resonant condition.

Original languageEnglish
Pages (from-to)707-714
Number of pages8
JournalJournal of Fluorescence
Volume21
Issue number2
DOIs
StatePublished - 1 Mar 2011

Keywords

  • Enhanced fluorescence
  • Photobleaching
  • Photonic crystal

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