Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Drug Carriers of mPEG-PCL-graft-cellulose

Yung Tsung Chen, Chao Hsuan Chen, Ming Fa Hsieh, Ann Shireen Chan, Ian Liau, Wan Yu Tai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The amphiphilic block copolymers methoxypoly (ethylene glycol)-poly (å-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce watersoluble copolymers. Doxorubicin (DOX)-loaded nanoparticles were prepared by dialysis method and the sizes of nanoparticles were determined by dynamic light scattering (DLS) in solution. The size of the nanoparticles was in the range of 197.4 to 340.7 nm. Rhodamine 123 was used to probe the relative Pglycoprotein (P-gp) expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. Confocal laser scanning microscopy (CLSM) showed a difference between the fluorescence images of DOX-loaded micelles and free DOX. For a quantitative basis, flow cytometry was done on both cell lines treated with DOX-loaded micelles and free DOX to compare a difference in effect. The amount of DOX-loaded micelles in MCF-7/ADR increased compared to that in the MCF-7/WT cells. This gives insight into how the micellar system developed in this study overcomes the multidrug resistance (MDR) effect.

Original languageEnglish
Title of host publication13th International Conference on Biomedical Engineering - ICBME 2008
Pages1224-1227
Number of pages4
DOIs
StatePublished - 1 Dec 2009
Event13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore
Duration: 3 Dec 20086 Dec 2008

Publication series

NameIFMBE Proceedings
Volume23
ISSN (Print)1680-0737

Conference

Conference13th International Conference on Biomedical Engineering, ICBME 2008
CountrySingapore
Period3/12/086/12/08

Keywords

  • P-glycoprotein
  • methoxy-poly (ethylene glycol)
  • multidrug resistance
  • poly (å-caprolactone)

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    Chen, Y. T., Chen, C. H., Hsieh, M. F., Chan, A. S., Liau, I., & Tai, W. Y. (2009). Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Drug Carriers of mPEG-PCL-graft-cellulose. In 13th International Conference on Biomedical Engineering - ICBME 2008 (pp. 1224-1227). (IFMBE Proceedings; Vol. 23). https://doi.org/10.1007/978-3-540-92841-6_300