Observation of microarray DNA hybridization using surface plasmon resonance phase-shift interferometry

Shean-Jen Chen*, C. Y. Tsou, Y. K. Chen, Y. T. Su

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review


Surface plasmon resonance phase-shift interferometry (SPR-PSI) is a novel technique which combines SPR and modified Mach-Zehnder phase-shifting interferometry to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable real-time quantitative information. Current SPR-PSI imaging systems measure the spatial phase variation caused by tiny biomolecular changes on the sensing interface by means of a five-step phase reconstruction algorithm and a novel multichannel least mean squares (MLMS) phase unwrapping algorithm. The SPR-PSI imaging system has an enhanced detection limit of 2.5 × 10-7 refraction index change, a long-term phase stability of π/100 in 30 minutes, and a spatial phase resolution of π/500 with a lateral resolution of 10 μm. This study successfully demonstrates the kinetic and label-free observation of 5-mer DNA microarray hybridization.

Original languageEnglish
Pages (from-to)80-87
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 16 Nov 2004
EventPlasmonics in Biology and Medicine - San Jose, CA, United States
Duration: 26 Jan 200427 Jan 2004


  • DNA hybridization
  • Imaging system
  • Microarray
  • Phase unwrapping
  • Phase-shifting interferometry
  • Surface plasmon resonance

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