Novel pyrene containing monomeric and dimeric supramolecular AIEE active nano-probes utilized in selective "off-on" trivalent metal and highly acidic pH sensing with live cell applications

Muthaiah Shellaiah, Turibius Simon, Venkatesan Srinivasadesikan, Chein Ming Lin, Kien-Wen Sun*, Fu-Hsiang Ko, Ming-Chang Lin, Hong-Cheu Lin

*Corresponding author for this work

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Two novel pyrene containing monomeric and dimeric Schiff base derivatives PCS1 and PCS2 have been synthesized via one-pot reaction and their nano-J-type aggregation induced emission enhancement (AIEE) was well demonstrated using UV-Vis/PL, transmission electron microscopy (TEM), dynamic light scattering (DLS), time resolved photoluminescence (TRPL), and live cell imaging studies. In contrast to PCS2, PCS1 in CH3CN exhibits fluorescence "off-on" sensor selectivity towards transition trivalent metal ions (Fe3+, Cr3+ and Al3+) among other metals, via PET inhibition with excimer PCS1-PCS1∗ formation. The 2 : 1 stoichiometry of PCS1⋯M3+ (M = Fe/Cr/Al) sensor complexes was calculated from Job's plots based on their PL titrations. In addition, the binding sites of PCS1⋯M3+ sensor complexes were well recognised from the 1H NMR titrations and supported by ESI(+ve) mass and FTIR analysis. Additionally, fluorescence reversibilities of PCS1⋯M3+ were observed via consequent addition of M3+ ions and PMDTA, respectively. Furthermore, the detection limits (LODs) and the association constant (Ka) values of PCS1⋯M3+ complexes were calculated using standard deviation and linear fittings. Likewise, quantum yield (Pdbl) measurements, TEM analysis, determination of the effect of pH, density functional theory (DFT) and time resolved photoluminescence (TRPL) studies were performed for the PCS1⋯M3+ sensor complexes. More importantly, confocal fluorescence microscopy imaging of Raw264.7 cells showed that PCS1 could be used as an effective fluorescent probe for detecting transition trivalent metal ions (Fe3+, Cr3+, and Al3+) in living cells. Impressively, both PCS1 and PCS2 showed "off-on" sensing at highly acidic pH values (1-3) with live cell applications.

Original languageEnglish
Pages (from-to)2056-2071
Number of pages16
JournalJournal of Materials Chemistry C
Volume4
Issue number10
DOIs
StatePublished - 14 Mar 2016

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