Novel microchip for in situ TEM imaging of living organisms and bio-reactions in aqueous conditions

Kuo Liang Liu, Chien Chen Wu, Ying Jung Huang, Hwei-Ling Peng, Hwan You Chang, Pin Chang, Long Hsu, Tri Rung Yew*

*Corresponding author for this work

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

A novel and disposable microchip (K-kit) with SiO2 nano-membranes was developed and used as a specimen kit for in situ imaging of living organisms in an aqueous condition using transmission electron microscopy (TEM) without equipment modification. This K-kit enabled the successful TEM observation of living Escherichia coli cells and the tellurite reduction process in Klebsiella pneumoniae. The K. pneumoniae and Saccharomyces cerevisiae can stay alive in K-kit after continuous TEM imaging for up to 14 s and 42 s, respectively. Besides, different tellurite reduction profiles in cells grown in aerobic and anaerobic environments can be clearly revealed. These results demonstrate that the K-kit developed in this paper can be useful for observing living organisms and monitoring biological processes in situ.

Original languageEnglish
Pages (from-to)1915-1921
Number of pages7
JournalLab on a Chip
Volume8
Issue number11
DOIs
StatePublished - 1 Jan 2008

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