Normalization of flow-in velocity for improving the evaluation on cell deformability

Chia-Hung Tsai, Shinya Sakuma, Fumihito Arai, Makoto Kaneko

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

A method for improving the evaluation of cell deformability is proposed. Cell deformability can be evaluated by the ratio between the cell velocities before and after entering the narrow throat of a microchannel. However, the flow-in velocity, the cell velocity before entering the throat, varies with respect to the location of the cell because of the laminar flow inside the microchannel. To compensate such velocity variation caused by different locations, we obtain a mean velocity by normalizing the velocity profile, and utilize this mean velocity for evaluating cell deformability. Experimental study on human red blood cells is performed. By implementing a high-speed vision system, we acquire the information of the location and velocity of every single cell through the narrow throat. The acquired data are processed by the proposed method. We compare both the results with and without the normalization, and find that the the proposed method effectively improve the consistency and accuracy of the evaluation.

Original languageEnglish
Title of host publication2013 IEEE International Conference on Mechatronics and Automation, IEEE ICMA 2013
Pages261-266
Number of pages6
DOIs
StatePublished - 25 Nov 2013
Event2013 10th IEEE International Conference on Mechatronics and Automation, IEEE ICMA 2013 - Takamastu, Japan
Duration: 4 Aug 20137 Aug 2013

Publication series

Name2013 IEEE International Conference on Mechatronics and Automation, IEEE ICMA 2013

Conference

Conference2013 10th IEEE International Conference on Mechatronics and Automation, IEEE ICMA 2013
CountryJapan
CityTakamastu
Period4/08/137/08/13

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