Nonserine esterases from rat liver cytosol

Dong Hyun Kim*, Yuh-Shyong Yang, William B. Jakoby

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

An effort to identify the major general esterases of rat liver cytosol that are insensitive to the serine esterase inhibitor paraoxon (diethyl 4-nitrophenyl phosphate) has led to the isolation of a dozen enzymes. Four of these are electrophoretically homogeneous. Although purified on the basis of their hydrolytic activity toward 4-nitrophenyl acetate, each of the enzymes has a very broad and overlapping substrate specificity for aromatic esters. Thiol esters serve as substrates but, within the limits of the methods used, amides are not hydrolyzed.

Original languageEnglish
Pages (from-to)19-27
Number of pages9
JournalProtein Expression and Purification
Volume1
Issue number1
DOIs
StatePublished - 1 Jan 1990

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