Nondestructive micro-patterning of proteinous occlusion bodies in water by femtosecond laser-induced mechanical force

Yoichiroh Hosokawa*, Takahiro Kaji, Chisa Shukunami, Yuji Hiraki, Eiji Kotani, Hajime Mori, Hiroshi Masuhara

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Some insect virus produces proteinous occlusion bodies named polyhedra, on which it is possible to immobilize functional proteins, such as green fluorescent proteins, antibodies, and growth factors, with keeping their biological activity. In this work, several kinds of polyhedra were micro-patterned by applying a mechanical force induced by femtosecond laser irradiation of a water buffer. In the printing process, since the laser is not directly irradiated on polyhedra, damages of polyhedra due to dryness and photothermal and photochemical reactions will be suppressed. The ability of the pattering was demonstrated by micro-sized checkerboard pattern, on which polyhedra with and without occluding enhanced green fluorescent protein were sorted. Furthermore, it was indicated that polyhedra are available as a scaffold of animal cell. This patterning method will be a promising technique to realize a microdevice in which functions of cell are artistically activated.

Original languageEnglish
Pages (from-to)105-111
Number of pages7
JournalBiomedical Microdevices
Volume9
Issue number2
DOIs
StatePublished - 1 Apr 2007

Keywords

  • Cell adhesion
  • Cell patterning
  • Femtosecond laser
  • Green fluorescent protein
  • Laser manufacturing
  • Polyhedra
  • Protein microarrays
  • Proteinous occlusion body

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