Non-destructive micro-patterning of protein crystals by focused femtosecond laser

Y. Hosokawa*, T. Kaji, Y. Hiraki, H. Mori, Hiroshi Masuhara

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations


Micro-patterning of proteins has been attracted much attention as a potential technique to realize bio-microdevice. In this work, as a new method to realize non-destructive micro-patterning of proteins, laser transfer printing for a μm-sized protein crystal was developed by utilizing focused femtosecond laser. The micro-patterning was performed to transfer the protein crystal which was adhered on a source substrate to a target substrate which was underlaid on the source substrate. An 800-nm femtosecond laser was focused in a water between the source and target substrates on an inverted microscope with a 100x objective lens. When the laser focal point was scanned at the position with distance of a few μm far from the source substrate, the protein crystals were detached by a shockwave and cavitation bubble generation at the circumstance of the focal point and transferred to the target substrate forming a line pattern. The line width of the protein crystal was a few tens μm with the scanning speed of 90 μm/sec. Furthermore, multi-patterning of several kinds of protein crystals was realized by this method. The pattering resolution is comparable or better than that by another multi-material transfer printing, such as ink jet printing, micro-printing, and laser direct writing.

Original languageEnglish
Article number610805
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 1 May 2006
EventCommercial and Biomedical Applications of Ultrafast Lasers VI - San Jose, CA, United States
Duration: 17 Jan 200619 Jan 2006


  • Animal cell
  • Crystal
  • Femtosecond laser ablation
  • Laser transfer printing
  • Micro-patterning
  • Protein

Fingerprint Dive into the research topics of 'Non-destructive micro-patterning of protein crystals by focused femtosecond laser'. Together they form a unique fingerprint.

Cite this