Non-contact type time-domain fluorescence diffuse optical tomography for quantitative analysis of fluorophores

Goro Nishimura, Daisuke Furukawa, Kamlesh Awasthi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A non-contact type time-domain system for the fluorescence diffuse optical tomography was designed. The system is evaluated by a phantom with a fluorescence target. The contamination of the non-specific scattering superimposed on the excitation profile but it could be reduced with closely locating the detection fiber to the surface (̃1 mm). Next, we analyzed the contamination in the temporal profiles with an Intralipid solution phantom with a fluorescent target. The contamination to the excitation profile is not clearly observed but that to the fluorescence is strong with a short distance between the excitation source and detection. Finally, we have concluded that a larger distance of source and detector yields better fluorescence sensitivity because the background is limiting the fluorescence detection. On the other hand, the signal quality depends on the statistics and thus the optimum range of the distance comes around 30 mm. Finally, this research gives the idea for the design of the source and detection configuration.

Original languageEnglish
Title of host publicationOptical Tomography and Spectroscopy of Tissue X
DOIs
StatePublished - 2013
EventOptical Tomography and Spectroscopy of Tissue X - San Francisco, CA, United States
Duration: 3 Feb 20136 Feb 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8578
ISSN (Print)1605-7422

Conference

ConferenceOptical Tomography and Spectroscopy of Tissue X
CountryUnited States
CitySan Francisco, CA
Period3/02/136/02/13

Keywords

  • fluorescence diffuse optical tomography
  • molecular imaging
  • time-resolved method

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