New water-soluble highly selective fluorescent chemosensor for Fe (III) ions and its application to living cell imaging

Shi Rong Liu, Shu-Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

A new rhodamine-based chemosensor exhibits excellent selectivity for Fe 3+ ions over a wide range of tested metal ions Ag +, Al 3+, Ca 2+, Cd 2+, Co 2+, Cr 3+, Cu 2+, Fe 2+, Hg 2+, Mg 2+, Mn 2+, Ni 2+, Pb 2+, and Zn 2+ in an aqueous solution. The binding of Fe 3+ to chemosensor 1 produces an absorption band at 564 nm and an emission band at 588 nm because Fe 3+-binding induces ring-opening of the spirolactam in 1. The binding ratio of the 1-Fe 3+ complexes was determined to be 1:1 according to a Job plot. The association constant (K a) of Fe 3+ binding in chemosensor 1 was 6.9 × 10 3 M -1. The maximum fluorescence enhancement caused by Fe 3+ binding in chemosensor 1 occurred at a pH range of 6-7.5. The fluorescence microscopy experiments in this study demonstrated that chemosensor 1 can be used as a fluorescent probe for detecting Fe 3+ in living cells.

Original languageEnglish
Pages (from-to)1110-1116
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume171-172
DOIs
StatePublished - 1 Aug 2012

Keywords

  • Imaging agents
  • Iron
  • Rhodamine
  • Sensors

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