NEURITE GROWTH COULD BE IMPAIRED BY ETFDH MUTATION BUT RESTORED BY MITOCHONDRIAL COFACTORS

Wen-Liang Chen, Yen-Fong Lin, Ting Yuan Liu, Cheng Chang Chang, Bai-Hsiun Chen, Ichizo Nishino, Yuh-Jyh Jong

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Introduction: c. 250G>A (p.Ala84Thr) in ETFDH is the most common mutation that causes later-onset multiple acyl-coenzyme A dehydrogenase deficiency (MADD) in the southern Chinese population. No functional study has targeted this mutation. Methods: Using cells expressing ETFDH-wild-type (WT) or ETFDH-mutant (p.Ala84Thr), reactive oxygen species (ROS) production and neurite length were analyzed, followed by pathomechanism exploration and drug screening. Results: Increased ROS production and marked neurite shortening were observed in the cells expressing the ETFDH-mutant, compared with WT. Further studies demonstrated that suberic acid, an accumulated intermediate metabolite in MADD, could significantly impair neurite outgrowth of NSC34 cells, but neurite shortening could be restored by supplementation with carnitine, riboflavin, or Coenzyme Q10. Conclusions: Neurite shortening caused by the c. 250G>A mutation in ETFDH suggests that neural defects could be underdiagnosed in human patients with MADD. This impairment might be treatable with mitochondrial cofactor supplementation.
Original languageEnglish
Pages (from-to)479-485
Number of pages7
JournalMuscle & Nerve
Volume56
Issue number3
DOIs
StatePublished - Sep 2017

Keywords

  • carnitine; coenzyme Q10; ETFDH; lipid storage myopathy; multiple acyl-coenzyme A dehydrogenase deficiency; neurite shortening; riboflavin
  • ELECTRON-TRANSFER FLAVOPROTEIN; COA DEHYDROGENASE-DEFICIENCY; ACIDEMIA TYPE-II; UBIQUINONE OXIDOREDUCTASE; OXIDATION DEFECTS; ALPHA-SUBUNIT; MYOPATHY; DYSFUNCTION; SUPEROXIDE; COENZYME

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