Multiplexed immunoassays for the analysis of breast cancer biopsies

Nicole Schneiderhan-Marra*, Georg Sauer, Cornelia Kazmaier, Hsin-Yun Hsu, Karin Koretz, Helmut Deissler, Thomas O. Joos

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Within the last decade, protein microarray technology has been successfully used for the simultaneous quantification of target proteins from minimal amounts of samples in basic and applied proteome research. The robustness and appropriate sensitivity of these miniaturized assays have been demonstrated and thus the transfer to routine and high-throughput applications is now possible. In this study, multiplexed bead-based sandwich immunoassays were used to determine the concentrations of 54 protein analytes, including HER 2 and the estrogen receptor, from ultrasound-guided large-core needle biopsies (LCNBs) from breast cancer patients. Expression levels for HER 2, estrogen receptors and progesterone receptors were also assessed by immunohistochemical routine staining, performed in the clinic on corresponding biopsy samples. The high concordance of the data sets generated with the bead-based protein arrays and by conventional immunohistochemical assessment of HER 2 and the estrogen receptor expressed by breast cancer cells present in the biopsies was demonstrated.

Original languageEnglish
Pages (from-to)3329-3338
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume397
Issue number8
DOIs
StatePublished - 1 Aug 2010

Keywords

  • Breast cancer
  • Estrogen receptor
  • HER 2
  • Large-core needle biopsy
  • Protein array

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    Schneiderhan-Marra, N., Sauer, G., Kazmaier, C., Hsu, H-Y., Koretz, K., Deissler, H., & Joos, T. O. (2010). Multiplexed immunoassays for the analysis of breast cancer biopsies. Analytical and Bioanalytical Chemistry, 397(8), 3329-3338. https://doi.org/10.1007/s00216-010-3873-7