Multiplex microsphere-based flow cytometric platforms for protein analysis and their application in clinical proteomics - From assays to results

Hsin-Yun Hsu*, Thomas O. Joos, Hisashi Koga

*Corresponding author for this work

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Advances in high-throughput screening, together with rapid progress in genomic and proteomic sciences, have considerably stimulated the development of a variety of biomarker discovery tools and have contributed to the improvement of clinical diagnosis. Major challenges include the obtaining of high-quality data sets based on assays that are rapid, reliable, and inexpensive, but still highly sensitive and easy to perform. The microsphere-based flow cytometric technology is a platform that is currently entering the diagnostic market. This article reviews the development and the applications of microsphere-based flow cytometric platforms in basic and applied proteomic research, and discusses bead-based assays used in clinical applications.

Original languageEnglish
Pages (from-to)4008-4019
Number of pages12
JournalElectrophoresis
Volume30
Issue number23
DOIs
StatePublished - 1 Dec 2009

Keywords

  • Clinical proteomics
  • Diagnostics
  • Microsphere-based flow cytometric platforms
  • Multiplexed assay
  • Suspension microarray

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