Multiphoton imaging of dye penetration dynamics in tissue sections

Po Hang Tseng, Shu Jen Chiang, Shean Jen Chen, Chen Yuan Dong*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Rapid and in-depth labeling biological tissue samples is invaluable for studying tissue organization in three-dimensions. The current strategy in finding optimal labeling parameters for dye molecules through tissues often requires trial and error testing. Difficulty in establishing a standard approach is due to lack of information on the diffusion parameters of dye through tissues. In this study we investigate the temporal progression of dye penetration in different tissue types. By the combination of multiphoton imaging of labeled tissues and application of the theoretical model of diffusion with well-defined boundary conditions, we aim to arrive at systematic parameters for optimizing dye penetration. Determination of the effective diffusion coefficient of the dyes used will then be useful for further application.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XX
EditorsAmmasi Periasamy, Peter T. C. So, Karsten Konig, Karsten Konig
ISBN (Electronic)9781510632516
StatePublished - 1 Jan 2020
EventMultiphoton Microscopy in the Biomedical Sciences XX 2020 - San Francisco, United States
Duration: 2 Feb 20204 Feb 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMultiphoton Microscopy in the Biomedical Sciences XX 2020
CountryUnited States
CitySan Francisco


  • 3-D Imaging
  • Dye Penetration
  • Multiphoton Microscopy

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