Multiphoton imaging and quantification of tissue glycation

Ara A. Ghazaryan, Jo Ya Tseng, Wen Lo, Yang Fang Chen, Vladimir Hovhannisyan, Shean-Jen Chen, Hsin Yuan Tan*, Chen Yuan Dong

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations


In this work, we applied multi-photon microscopy to image and characterize the extent of tissue glycation. Specifically, features of multi-photon autofluorescence (MPAF) and second harmonic generation (SHG) images were presented. Our study shows that multiphoton imaging is capable of providing qualitative and quantitative information of the extent of tissue glycation in ribose treated samples including bovine sclera and vessel and that this approach may be useful for clinical evaluation of advanced glycation endproducts (AGEs) formation.

Original languageEnglish
Title of host publicationOptical Biopsy IX
StatePublished - 21 Apr 2011
EventOptical Biopsy IX - San Francisco, CA, United States
Duration: 24 Jan 201126 Jan 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceOptical Biopsy IX
CountryUnited States
CitySan Francisco, CA


  • advanced glycation product
  • glycation
  • multiphoton microscopy
  • spectral imaging

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