Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy

Wen Lo*, Chih Ting Hsu, Tsung Rong Kuo, Chung Long Wu, Shu Jen Chiang, Sung Jan Lin, Shean-Jen Chen, Chia Chun Chen, Chen Yuan Dong

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Zinc oxide nanoparticles (ZnO NPs) are commonly used in sunscreens to reduce the risk of skin cancer by blocking ultraviolet radiation. ZnO NPs absorption through the transdermal route may not cause high health risk as inhalation or ingestion. However, in practical usage of sunscreens and cosmetics, ZnO NPs are topically applied to a large area of skin with long periods hence the potential absorption amount of ZnO NPs is still need to be concerned. Therefore, if the ZnO NPs are able the pass the barrier of normal skin, the pathways of transdermal delivery and the factors of enhancements become important issues. In this work, multiphoton microscopy provides us a non-invasive visualization of ZnO NPs in skin. Moreover, we quantitatively analyzed the enhancement of oleic acid and ethanol. Due to the fact that photoluminance of ZnO NPs spectrally overlaps autofluorence from skin stratum corneum (SC) and high turbidity of both ZnO NPs and SC, it is difficult to resolve the distribution of ZnO NPs in skin by using fluorescence microscopy. In this work, the second harmonic generation (SHG) signals from ZnO NPs which double the frequency of excitation source to characterize the delivery pathways and penetration depth in skin. Moreover, we quantitatively compare the ZnO NPs delivery efficiency in normal skin and in skins with three chemically enhancing conditions: ethanol, oleic acid and the combination of ethanol and oleic acid.

Original languageEnglish
Title of host publicationPhotonic Therapeutics and Diagnostics VI
DOIs
StatePublished - 1 Dec 2010
EventPhotonic Therapeutics and Diagnostics VI - San Francisco, CA, United States
Duration: 23 Jan 201025 Jan 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7548
ISSN (Print)1605-7422

Conference

ConferencePhotonic Therapeutics and Diagnostics VI
CountryUnited States
CitySan Francisco, CA
Period23/01/1025/01/10

Fingerprint Dive into the research topics of 'Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy'. Together they form a unique fingerprint.

  • Cite this

    Lo, W., Hsu, C. T., Kuo, T. R., Wu, C. L., Chiang, S. J., Lin, S. J., Chen, S-J., Chen, C. C., & Dong, C. Y. (2010). Monitoring chemically enhanced transdermal delivery of zinc oxide nanoparticles by using multiphoton microscopy. In Photonic Therapeutics and Diagnostics VI [75480P] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7548). https://doi.org/10.1117/12.843187