Molecular recognition between insulin and dextran encapsulated gold nanoparticles

Kai Chieh Lee, Hsiang Ling Chiang, Wei Ru Chiu, Yu-Chie Chen

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Insulin is a peptide hormone that can regulate the metabolism of carbohydrates and lipids. This hormone is closely related to glucose-uptake in cells and can control blood glucose levels. Dextran is a polysaccharide composed of glucose units. In this study, we discovered that dextran-encapsulated gold nanoparticles (AuNPs@Dextran) and nanoclusters (AuNCs@Dextran) can be used to recognize insulin. The dissociation constant of insulin toward AuNPs@Dextran was estimated to be ∼5.3 × 10-6  M. The binding site on insulin toward the dextran on the nanoprobes was explored as well. It was found that the sequence of numbers 1-22 on the insulin B chain can interact with the dextran encapsulated nanoprobes. Additionally, we also demonstrated that the dextran-encapsulated nanoprobes could be used as concentration probes to selectively enrich trace amounts of insulin (∼1 pM) from serum samples.

Original languageEnglish
Pages (from-to)528-535
Number of pages8
JournalJournal of molecular recognition : JMR
Volume29
Issue number11
DOIs
StatePublished - 1 Nov 2016

Keywords

  • dextran
  • glucose
  • gold nanoclusters
  • gold nanoparticles
  • insulin

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