Molecular genetic analysis reveals that a nonribosomal peptide synthetase-like (NRPS-like) gene in Aspergillus nidulans is responsible for microperfuranone biosynthesis

Hsu Hua Yeh, Yi Ming Chiang, Ruth Entwistle, Manmeet Ahuja, Kuan Han Lee, Kenneth S. Bruno, Tung-Kung Wu, Berl R. Oakley, Clay C C Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Genome sequencing of Aspergillus species including Aspergillus nidulans has revealed that there are far more secondary metabolite biosynthetic gene clusters than secondary metabolites isolated from these organisms. This implies that these organisms can produce additional secondary metabolites, which have not yet been elucidated. The A. nidulans genome contains 12 nonribosomal peptide synthe-tase (NRPS), one hybrid polyketide synthase/NRPS, and 14 NRPS-like genes. The only NRPS-like gene in A. nidulans with a known product is tdiA, which is involved in terrequi-none A biosynthesis. To attempt to identify the products of these NRPS-like genes, we replaced the native promoters of the NRPS-like genes with the inducible alcohol dehydroge-nase (alcA) promoter. Our results demonstrated that induction of the single NRPS-like gene AN3396.4 led to the enhanced production of microperfuranone. Furthermore, heterologous expression of AN3396.4 in Aspergillus niger confirmed that only one NRPS-like gene, AN3396.4, is necessary for the production of microperfuranone.

Original languageEnglish
Pages (from-to)739-748
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume96
Issue number3
DOIs
StatePublished - 1 Nov 2012

Keywords

  • Aspergillus nidulans
  • Biosynthesis
  • Microperfuranone
  • Nonribosomal peptide synthetase-like

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