Modulation of macrophage differentiation and activation by decoy receptor 3

Yung Chi Chang, Tsui Ling Hsu, Hsi Hsien Lin, Chung Ching Chio, Allen W. Chiu, Nien Jung Chen, Chi Hung Lin, Shie Liang Hsieh*

*Corresponding author for this work

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

Decoy receptor 3 (DcR3) is a soluble receptor of the tumor necrosis factor receptor superfamily and is readily detected in certain cancer patients. Recently, we demonstrated that DcR3.Fc-treated dendritic cells skew T cell responses to a T helper cell type 2 phenotype. In this study, we further asked its ability to modulate CD14+ monocyte differentiation into macrophages induced by macrophage-colony stimulating factor in vitro. We found that DcR3.Fc was able to modulate the expression of several macrophage markers, including CD14, CD16, CD64, and human leukocyte antigen-DR. In contrast, the expression of CD11c, CD36, CD68, and CD206 (mannose receptor) was not affected in the in vitro culture system. Moreover, phagocytic activity toward immune complexes and apoptotic bodies as well as the production of free radicals and proinflammatory cytokines in response to lipopolysaccharide were impaired in DcR3.Fc-treated monocyte-derived macrophages. This suggests that DcR3.Fc might have potent, suppressive effects to down-regulate the host-immune system.

Original languageEnglish
Pages (from-to)486-494
Number of pages9
JournalJournal of Leukocyte Biology
Volume75
Issue number3
DOIs
StatePublished - Mar 2004

Keywords

  • Apoptosis
  • Inflammation
  • Phagocytosis
  • Tumor necrosis factor

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