Micropatterning of perfluoroalkyl self-assembled monolayers for arraying proteins and cells on chips

Atsushi Kira*, Kazunori Okano, Yoichiroh Hosokawa, Akira Naito, Koh Fuwa, Jyunpei Yuyama, Hiroshi Masuhara

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Organosilane self-assembled monolayers (SAMs) with perfluoroalkyl groups (R f ) on glass surfaces were used for arraying proteins and cells on chips. Quartz crystal microbalance measurements confirmed the inhibition of protein adsorption on R f -SAM-modified surfaces and showed efficient adsorption on hydroxyl-, carboxyl-, and amino group-modified surfaces. The characteristics of R f -modified surfaces were evaluated using solvent contact angle measurement and Fourier transform infrared (FTIR) spectroscopy. The R f surface was highly water- and oil-resistant, as inferred from the contact angles of water, oleic acid, and hexadecane. Specific peaks of IR spectra were detected in the region from 1160 to 1360 cm -1 . Etching with dry plasma completely exfoliated the R f -SAM, exposing the underlying intact glass surface. Modification conditions were optimized using contact angle and FTIR measurements. After dry plasma processing, the contact angles of all solvents became undetectable, and the IR peaks disappeared. Micrometer scale protein and cell patterns can be fabricated using the proposed method. Protein adsorption on micropatterned R f -SAM-modified chips was evaluated using fluorescence analysis; protein adsorption was easily controlled by patterning R f -SAM. PC12 and HeLa cells grew well on micropatterned R f -SAM-modified chips. Micropatterning of R f -SAM by dry plasma treatment with photolithography is useful for the spatial arrangement of proteins and cells.

Original languageEnglish
Pages (from-to)7647-7651
Number of pages5
JournalApplied Surface Science
Issue number17
StatePublished - 15 Jun 2009


  • Cell patterning
  • Chemical surface modification
  • Microarray
  • Microfabrication
  • Protein patterning
  • Two-dimensional patterning of self-assemble monolayer

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