Microengineered heterogeneous substrates for cell culture by electro-microfluidics

Min Yu Chiang, Yao Wen Hsu, Hsin Yi Hsieh, San-Yuan Chen, Shih Kang Fan*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Cultivating cells on a heterogeneous substrate prepared by assembly of hydrogels and arrangement of microparticles using electric manners on an electro-microfluidic (EMF) device are reported. We demonstrated a multifunctional integrated EMF device, combining (1) electrical manipulations of dielectrophoresis (DEP) and electrowetting-on-dielectric (EWOD) and (2) UV crosslinking technique, in order to manipulate, assemble, and fabricate complex microengineered hydrogels. With the ability to drive and assemble the hydrogel droplets and to arrange the microparticles within the hydrogel droplets, the highly programmable culture substrate provides an approach to tissue engineering, cell-cell interaction, stem cell differentiation studies. Poly(ethylene glycol) diacrylate (PEG-DA) and gelatin methacrylate (GelMA) were manipulated to form heterogeneous substrates for neonatal mouse cardiomyocytes culture.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages96-98
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 1 Jan 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 26 Oct 201430 Oct 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period26/10/1430/10/14

Keywords

  • Dielectrophoresis
  • Electro-microfluidics
  • Electrowetting
  • Hydrogel

Fingerprint Dive into the research topics of 'Microengineered heterogeneous substrates for cell culture by electro-microfluidics'. Together they form a unique fingerprint.

Cite this