Microcontroller-Assisted Compensation of Adenosine Triphosphate Levels: Instrument and Method Development

Jie Bi Hu, Ting Ru Chen, Yu-Chie Chen, Pawel L. Urban*

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

In order to ascertain optimum conditions for biocatalytic processes carried out in vitro, we have designed a bio-opto-electronic system which ensures real-time compensation for depletion of adenosine triphosphate (ATP) in reactions involving transfer of phosphate groups. The system covers ATP concentration range of 2-48 μM. The report demonstrates feasibility of the device operation using apyrase as the ATP-depleting enzyme.

Original languageEnglish
Article number8135
JournalScientific reports
Volume5
DOIs
StatePublished - 30 Jan 2015

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