Microarray analysis of microRNA expression in renal clear cell carcinoma

Y. Huang, Y. Dai*, J. Yang, Ting-Wen Chen, Y. Yin, M. Tang, C. Hu, L. Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

Aim: To explore the microRNA (miRNA) expression in renal clear cell carcinoma (RCCC). Methods: We compared the miRNA expression profiles in 11 pairs of RCCC and adjacent nontumorous tissue (NT) from 11 RCCC patients, using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. To verify microarray results, Northern blotting was carried out on 5 randomly selected miRNAs. Results: Totally 81 miRNAs were identified valid expression in RCCC samples, 48 of which specifically detected in RCCC samples, 17 of which detected downregulated in RCCC compared to NT sample, 2 upregulated and 14 without significant difference. MiRNAs in RCCC tissues exhibit an overall higher expression level than NT tissue. The chip results were confirmed by northern blot analysis. Conclusion: Our study may help to clarify the molecular mechanisms involved in the pathogenesis of RCCC, and miRNAs potentially serve as a novel diagnostic biomarker of RCCC. Crown

Original languageEnglish
Pages (from-to)1119-1123
Number of pages5
JournalEuropean Journal of Surgical Oncology
Volume35
Issue number10
DOIs
StatePublished - 1 Oct 2009

Keywords

  • Human
  • Microarray
  • MicroRNA
  • Renal cell carcinoma

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