Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays

Chie Hosokawa; Suguru N. Kudoh; Mariko Suzuki; Ai Kiyohara; Yoichiroh Hosokawa; Kazunori Okano; Hiroshi Masuhara; Takahisa Taguchi

doi:10.1007/s00339-010-5836-4

Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays

Chie Hosokawa^*, Suguru N. Kudoh, Mariko Suzuki, Ai Kiyohara, Yoichiroh Hosokawa, Kazunori Okano, Hiroshi Masuhara, Takahisa Taguchi

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article

10 Scopus citations

Abstract

We propose and demonstrate micro-channel fabrication by femtosecond-laser processing to arrange neuronal cells on multi-electrode arrays (MEAs). When neuronal cells were seeded in the micro-channels on MEAs, they elongated their neurites along the channel and finally formed the network patterned on MEAs. The spontaneous electrical activity of the neuronal networks on MEAs was evaluated by using electrophysiological systems, indicating that patterned network was functionally activated along the channels. The present method is applicable to analyze network dynamics of living neuronal cells patterned artificially.

Original language	English
Pages (from-to)	423-428
Number of pages	6
Journal	Applied Physics A: Materials Science and Processing
Volume	101
Issue number	2
DOIs	https://doi.org/10.1007/s00339-010-5836-4
State	Published - 1 Nov 2010

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Hosokawa, C., Kudoh, S. N., Suzuki, M., Kiyohara, A., Hosokawa, Y., Okano, K., Masuhara, H., & Taguchi, T. (2010). Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays. Applied Physics A: Materials Science and Processing, 101(2), 423-428. https://doi.org/10.1007/s00339-010-5836-4

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Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays. / Hosokawa, Chie; Kudoh, Suguru N.; Suzuki, Mariko; Kiyohara, Ai; Hosokawa, Yoichiroh; Okano, Kazunori; Masuhara, Hiroshi; Taguchi, Takahisa.

In: Applied Physics A: Materials Science and Processing, Vol. 101, No. 2, 01.11.2010, p. 423-428.

Research output: Contribution to journal › Article

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