Measurement of the adhesive force between a single Klebsiella pneumoniae type 3 fimbria and collagen IV using optical tweezers

Bo Jui Chang, Ying Jung Huang, Chia Han Chan, Long Hsu*, Hwei-Ling Peng, Hwan You Chang, Tri Rung Yew, Cheng Hsien Liu, Sien Chi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Type 3 fimbriae are important adhesive filaments that assist Klebsiella pneumoniae to establish an infection. Different MrkD adhesin variants on the fimbriae are known to display distinct adherence capability for the bacteria to bind extracellular matrix proteins, although the difference has not been determined physically. For this reason, the adhesive force between type 3 fimbriae and collagen IV were measured using optical tweezers. The measured force data displayed a periodic histogram thus Fourier analysis was applied to group it to extract the adhesive force of a single molecular pair. Specifically, we showed that grouping should begin with an offset at the first half of the period. Finally, we first present the adhesive force between each mrkDV2-, mrkDV3-, and mrkDV4-expressed fimbriae and collagen IV is 2.03, 3.79, and 2.87 pN, respectively. This result can be referred to further research on mrkD allelic effect on bacteria infection.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume350
Issue number1
DOIs
StatePublished - 10 Nov 2006

Keywords

  • Adhesive force
  • Fourier analysis
  • Grouping offset
  • Klebsiella pneumoniae
  • Optical tweezers
  • Type 3 fimbriae

Fingerprint Dive into the research topics of 'Measurement of the adhesive force between a single Klebsiella pneumoniae type 3 fimbria and collagen IV using optical tweezers'. Together they form a unique fingerprint.

Cite this