Measurement of macrophage adhesion by optical tweezers with backward-scattered detection

Sung Yang Wei*, Yi Jr Su, Po Chen Shih, Shih Mo Yang, Long Hsu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Macrophages are members of the leukocyte family. Tissue damage causes inflammation and release of vasoactive and chemotactic factors, which trigger a local increase in blood flow and capillary permeability. Then, leukocytes accumulate quickly to the infection site. The leukocyte extravasation process takes place according to a sequence of events that involve tethering, activation by a chemoattractant stimulus, adhesion by integrin binding, and migrating to the infection site. The leukocyte extravasation process reveals that adhesion is an important part of the immune system. Optical tweezers have become a useful tool with broad applications in biology and physics. In force measurement, the trapped bead as a probe usually uses a polystyrene bead of 1 μm diameter to measure adhesive force between the trapped beads and cell by optical tweezers. In this paper, using the ray-optics model calculated trapping stiffness and defined the linear displacement ranges. By the theoretical values of stiffness and linear displacement ranges, this study attempted to obtain a proper trapped particle size in measuring adhesive force. Finally, this work investigates real-time adhesion force measurements between human macrophages and trapped beads coated with lipopolysaccharides using optical tweezers with backscattered detection.

Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation VII
DOIs
StatePublished - 27 Oct 2010
EventOptical Trapping and Optical Micromanipulation VII - San Diego, CA, United States
Duration: 1 Aug 20105 Aug 2010

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7762
ISSN (Print)0277-786X

Conference

ConferenceOptical Trapping and Optical Micromanipulation VII
CountryUnited States
CitySan Diego, CA
Period1/08/105/08/10

Keywords

  • Backscattered detection
  • Force measurements
  • Lipopolysaccharides
  • Macrophage
  • Optical tweezers

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