Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag

Evangelos Gatzogiannis, Zhixing Chen, Lu Wei, Richard Wombacher, Ya-Ting Kao, Grygorii Yefremov, Virginia W. Cornish, Wei Min*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.

Original languageEnglish
Pages (from-to)8694-8696
Number of pages3
JournalChemical Communications
Volume48
Issue number69
DOIs
StatePublished - 7 Sep 2012

Fingerprint Dive into the research topics of 'Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag'. Together they form a unique fingerprint.

Cite this