Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7

Yi Ju Liu, Da Jeng Yao*, Hwan You Chang, Chien Ming Liu, Chih Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

Original languageEnglish
Pages (from-to)558-565
Number of pages8
JournalBiosensors and Bioelectronics
Volume24
Issue number4
DOIs
StatePublished - 1 Dec 2008

Keywords

  • Biotin-streptavidin
  • Biotinlyted linker
  • DNA detection
  • Magnetic bead
  • Quantum dots

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