Lysosomal targeting of phafin1 mediated by Rab7 induces autophagosome formation

Wen Jie Lin, Chih Yung Yang, Li Li Li, Yung Hsiang Yi, Ke Wei Chen, Ying Chih Lin, Chin Chun Liu, Chi Hung Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Autophagy orchestrates programmed cell death via crossroads of complex vesicle trafficking including autophagosome and lysosome interaction. Phafin1, an endosome proteins composed of Pleckstrin homology (PH) and Fab1-YotB-Vac1p-EEA1 (FYVE) domain membrane-binding domains, is involved in caspase-independent apoptosis. We report here that the increased expression of phafin1 and its FYVE domain caused the formation of enlarged endosomes. Phafin1 also modulates the membrane density of certain receptors and participates in endocytosis and autophagy processes. The PH-domain of phafin1 is dispensable for lysosomal targeting. Moreover, the tail-domain of phafin1 provides lysosomal targeting signature and the ability to induce autophagy that is mediated by Rab7 signaling. The results suggest that in addition to its role in endosome transport, phafin1 is also involved in lysosomal targeting and autophagosome formation.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume417
Issue number1
DOIs
StatePublished - 6 Jan 2012

Keywords

  • Autophagosome
  • Endocytosis
  • FYVE domain
  • Lysosome
  • Phafin1
  • Rab7

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