Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave

Yung En Kuo; Cheng Chi Wu; Yoichiroh Hosokawa; Yasuyo Maezawa; Kazunori Okano; Hiroshi Masuhara; Fu Jen Kao

doi:10.1007/s00339-010-5927-2

Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave

Yung En Kuo, Cheng Chi Wu, Yoichiroh Hosokawa^*, Yasuyo Maezawa, Kazunori Okano, Hiroshi Masuhara, Fu Jen Kao

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

When an 800 nm femtosecond laser is tightly focused into cell culture medium a stress wave is generated at the laser focal point. Since the stress wave localizes in a few tens of μm, it is possible to locally stimulate single cells in vitro. In this work, several kinds of cultured mammalian cells, HeLa, PC12, P19CL6, and C2C12, were stimulated by the stress wave and the cell growth after the stress loading with the laser irradiation was investigated. In comparison with the control conditions, cell growth after the laser irradiation was enhanced for the cells of C2C12 and P19CL6, which can differentiate into myocytes, and suppressed for PC12 and HeLa cell lines. These results suggest a possibility of cell growth enhancement due to myogenic cells response to the femtosecond laser-induced stress.

Original language	English
Pages (from-to)	597-600
Number of pages	4
Journal	Applied Physics A: Materials Science and Processing
Volume	101
Issue number	4
DOIs	https://doi.org/10.1007/s00339-010-5927-2
State	Published - 1 Dec 2010

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title = "Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave",

abstract = "When an 800 nm femtosecond laser is tightly focused into cell culture medium a stress wave is generated at the laser focal point. Since the stress wave localizes in a few tens of μm, it is possible to locally stimulate single cells in vitro. In this work, several kinds of cultured mammalian cells, HeLa, PC12, P19CL6, and C2C12, were stimulated by the stress wave and the cell growth after the stress loading with the laser irradiation was investigated. In comparison with the control conditions, cell growth after the laser irradiation was enhanced for the cells of C2C12 and P19CL6, which can differentiate into myocytes, and suppressed for PC12 and HeLa cell lines. These results suggest a possibility of cell growth enhancement due to myogenic cells response to the femtosecond laser-induced stress.",

author = "Kuo, {Yung En} and Wu, {Cheng Chi} and Yoichiroh Hosokawa and Yasuyo Maezawa and Kazunori Okano and Hiroshi Masuhara and Kao, {Fu Jen}",

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T1 - Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave

AU - Kuo, Yung En

AU - Wu, Cheng Chi

AU - Hosokawa, Yoichiroh

AU - Maezawa, Yasuyo

AU - Okano, Kazunori

AU - Masuhara, Hiroshi

AU - Kao, Fu Jen

PY - 2010/12/1

Y1 - 2010/12/1

N2 - When an 800 nm femtosecond laser is tightly focused into cell culture medium a stress wave is generated at the laser focal point. Since the stress wave localizes in a few tens of μm, it is possible to locally stimulate single cells in vitro. In this work, several kinds of cultured mammalian cells, HeLa, PC12, P19CL6, and C2C12, were stimulated by the stress wave and the cell growth after the stress loading with the laser irradiation was investigated. In comparison with the control conditions, cell growth after the laser irradiation was enhanced for the cells of C2C12 and P19CL6, which can differentiate into myocytes, and suppressed for PC12 and HeLa cell lines. These results suggest a possibility of cell growth enhancement due to myogenic cells response to the femtosecond laser-induced stress.

AB - When an 800 nm femtosecond laser is tightly focused into cell culture medium a stress wave is generated at the laser focal point. Since the stress wave localizes in a few tens of μm, it is possible to locally stimulate single cells in vitro. In this work, several kinds of cultured mammalian cells, HeLa, PC12, P19CL6, and C2C12, were stimulated by the stress wave and the cell growth after the stress loading with the laser irradiation was investigated. In comparison with the control conditions, cell growth after the laser irradiation was enhanced for the cells of C2C12 and P19CL6, which can differentiate into myocytes, and suppressed for PC12 and HeLa cell lines. These results suggest a possibility of cell growth enhancement due to myogenic cells response to the femtosecond laser-induced stress.

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