TY - JOUR
T1 - Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave
AU - Kuo, Yung En
AU - Wu, Cheng Chi
AU - Hosokawa, Yoichiroh
AU - Maezawa, Yasuyo
AU - Okano, Kazunori
AU - Masuhara, Hiroshi
AU - Kao, Fu Jen
PY - 2010/12/1
Y1 - 2010/12/1
N2 - When an 800 nm femtosecond laser is tightly focused into cell culture medium a stress wave is generated at the laser focal point. Since the stress wave localizes in a few tens of μm, it is possible to locally stimulate single cells in vitro. In this work, several kinds of cultured mammalian cells, HeLa, PC12, P19CL6, and C2C12, were stimulated by the stress wave and the cell growth after the stress loading with the laser irradiation was investigated. In comparison with the control conditions, cell growth after the laser irradiation was enhanced for the cells of C2C12 and P19CL6, which can differentiate into myocytes, and suppressed for PC12 and HeLa cell lines. These results suggest a possibility of cell growth enhancement due to myogenic cells response to the femtosecond laser-induced stress.
AB - When an 800 nm femtosecond laser is tightly focused into cell culture medium a stress wave is generated at the laser focal point. Since the stress wave localizes in a few tens of μm, it is possible to locally stimulate single cells in vitro. In this work, several kinds of cultured mammalian cells, HeLa, PC12, P19CL6, and C2C12, were stimulated by the stress wave and the cell growth after the stress loading with the laser irradiation was investigated. In comparison with the control conditions, cell growth after the laser irradiation was enhanced for the cells of C2C12 and P19CL6, which can differentiate into myocytes, and suppressed for PC12 and HeLa cell lines. These results suggest a possibility of cell growth enhancement due to myogenic cells response to the femtosecond laser-induced stress.
UR - http://www.scopus.com/inward/record.url?scp=78650623297&partnerID=8YFLogxK
U2 - 10.1007/s00339-010-5927-2
DO - 10.1007/s00339-010-5927-2
M3 - Article
AN - SCOPUS:78650623297
VL - 101
SP - 597
EP - 600
JO - Applied Physics A: Materials Science and Processing
JF - Applied Physics A: Materials Science and Processing
SN - 0947-8396
IS - 4
ER -