Liposome-based polymer complex as a novel adjuvant:Enhancement of specifc antibody production and isotype switch

Chia Hung Chen, Yu Ling Lin, Yen Ku Liu, Pei Juin He, Ching Min Lin, Yi Han Chiu, Chang Jer Wu, Tian Lu Cheng, Shih Jen Liu, Kuang-Wen Liao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The aim of vaccination is to induce appropriate immunity against pathogens. Antibody-mediated immunity is critical for protection against many virus diseases, although it is becoming more evident that coordinated, multifunctional immune responses lead to the most effective defense. Specific antibody (Ab) isotypes are more efficient at protecting against pathogen invasion in different locations in the body. For example, compared to other Ab isotypes, immunoglobulin (Ig) A provides more protection at mucosal areas. In this study, we developed a cationic lipopolymer (liposome-polyethylene glycol-polyethyleneimine complex [LPPC]) adjuvant that strongly adsorbs antigens or immunomodulators onto its surface to enhance or switch immune responses. The results demonstrate that LPPC enhances uptake ability, surface marker expression, proinfammatory cytokine release, and antigen presentation in mouse phagocytes. In contrast to Freund's adjuvant, LPPC preferentially activates Th1-immunity against antigens in vivo. With lipopolysaccharides or CpG oligodeoxynucleotides, LPPC dramatically enhances the IgA or IgG2A proportion of total Ig, even in hosts that have developed Th2 immunities and high IgG1 serum titers. Taken together, the results demonstrate that the LPPC adjuvant not only increases the immunogenicity of antigens but also modulates host immunity to produce an appropriate Ab isotype by combining with immunomodulators.

Original languageEnglish
Pages (from-to)607-621
Number of pages15
JournalInternational Journal of Nanomedicine
Volume7
DOIs
StatePublished - 10 Feb 2012

Keywords

  • Adjuvant
  • Class switch
  • Immunomodulator
  • Liposome-PEG-PEI complex
  • Vaccine

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