Line-scanning detection instrument for photonic crystal enhanced fluorescence

Vikram Chaudhery, Meng Lu, Cheng-Sheng Huang, James Polans, Ruimin Tan, Richard C. Zangar, Brian T. Cunningham*

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A laser line-scanning instrument was developed to optimize the near-field enhancement capability of a onedimensional photonic crystal (PC) for excitation of surface-bound fluorophores. The excitation laser beam is shaped into an 8 μm × 1 mm line that is focused along the direction of the PC grating, while remaining collimated perpendicular to the grating. Such a beam configuration offers high excitation power density while simultaneously providing high resonant coupling efficiency from the laser to the PC surface. Using a panel of 21 immunofluorescence assays on the PC surface in a microarray format, the approach achieves an enhancement factor as high as 90-fold between on-resonance and off-resonance illumination. The instrument provides a capability for sensitive and inexpensive analysis of cancer biomarkers in clinical applications.

Original languageEnglish
Pages (from-to)2565-2567
Number of pages3
JournalOptics Letters
Volume37
Issue number13
DOIs
StatePublished - 1 Jul 2012

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    Chaudhery, V., Lu, M., Huang, C-S., Polans, J., Tan, R., Zangar, R. C., & Cunningham, B. T. (2012). Line-scanning detection instrument for photonic crystal enhanced fluorescence. Optics Letters, 37(13), 2565-2567. https://doi.org/10.1364/OL.37.002565