Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform

Hung Chun Lo, Hsin I. Hsiung, Surojit Chattopadhyay*, Hsieh Cheng Han, Chia Fu Chen, Leu-Jih Perng, Kuei Hsien Chen, Li Chyong Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10. nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms.

Original languageEnglish
Pages (from-to)2413-2418
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number5
DOIs
StatePublished - 15 Jan 2011

Keywords

  • Biosensor
  • DNA
  • Nanostructure
  • Surface enhanced Raman spectroscopy
  • Surface plasmon

Fingerprint Dive into the research topics of 'Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform'. Together they form a unique fingerprint.

  • Cite this