Label-free protein quantitation by dielectric spectroscopy of dual-frequency liquid crystal

Ching Min Lin, Po Chang Wu, Mon Juan Lee*, Wei Lee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


A dual-frequency-liquid-crystal (DFLC)-based biosensor was developed and its frequency-dependent dielectric properties were exploited to detect and quantitate a protein standard, bovine serum albumin (BSA). By analyzing the dielectric spectra of DFLC in the presence of BSA at various concentrations, we found that the difference in dielectric permittivity between the high- and low-frequency regimes is correlated to BSA concentration, thereby permitting a DFLC-based protein quantitative method. The dielectric properties of DFLCs are fundamental in the design of liquid crystal displays and fast-switching devices. Results from this study demonstrate the extended potential of the frequency-revertible dielectric anisotropy of DFLC in biosensing and protein quantitation.

Original languageEnglish
Pages (from-to)158-163
Number of pages6
JournalSensors and Actuators, B: Chemical
StatePublished - 1 Mar 2019


  • Bovine serum albumin
  • Dielectric spectroscopy
  • Dual-frequency liquid crystal
  • LC-based biosensor
  • Protein

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