Label-free detection of prostate specific antigen using a silicon nanobelt field-effect transistor

Chi Chang Wu, Tung Ming Pan, Chung Shu Wu, Li Chen Yen, Cheng Keng Chuang, See Tong Pang, Yuh-Shyong Yang, Fu-Hsiang Ko*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

In this study, we proposed a silicon nanobelt field-effect transistor (FET) to detect prostate specific antigen (PSA). The nanobelt FET device displayed n-channel depletion characteristics. The immobilization of prostate specific antibody (anti-PSA) molecules was attached onto the nanobelt FET surface by using the aldehyde groups of glutaraldehyde linked to the amino groups of 3-aminopropyltriethoxysilane (APTES). The shift in the drain current vs time curves of a nanobelt FET biosensor revealed that the electrical signal had a logarithmic relationship with respect to the concentration of the PSA, and detection capability was estimated in the 5 pg/mL level. To enhance the sensitivity of a nanobelt FET biosensor, this biosensor was designed by inserting arginine molecules between glutaraldehyde and APTES. Therefore, the detection capability of the developed sensor was extended to 50 fg/mL. Also, the relationship between the current shift and the logarithm of PSA concentration was exhibited linearity in the range 50 fg/mL - 500 pg/mL. The excellent electrical results of this label-free PSA nanobelt FET biosensor suggested that such biosensor might be potentially useful tools for biological research and future prostate cancer screening.

Original languageEnglish
Pages (from-to)4432-4442
Number of pages11
JournalInternational Journal of Electrochemical Science
Volume7
Issue number5
StatePublished - 2 Jul 2012

Keywords

  • Biosensor
  • Field-effect transistor
  • Label-free detection
  • Nanobelt
  • Prostate specific antigen

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