Iron oxide/niobium oxide core-shell magnetic nanoparticle-based phosphopeptide enrichment from biological samples for MALDI MS analysis

Hong Yi Lin, Wei Yu Chen, Yu-Chie Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The feasibility of using niobium oxide-coated magnetic nanoparticles (NPs) as affinity probes for selectively trapping phosphopeptides from peptide mixtures including tryptic digest of caseins, serum, and cell lysate was demonstrated in this study. Phosphopeptide enrichment was rapid when subjecting these systems to microwave heating for 1 min; the probe-target species, which were readily isolated through magnetic separation, were then analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Only signals for phosphopeptides were present in the resulting mass spectra. The detection limit for monophosphopeptide was as low as 5 fmol.

Original languageEnglish
Pages (from-to)215-223
Number of pages9
JournalJournal of Biomedical Nanotechnology
Volume5
Issue number2
DOIs
StatePublished - 1 Apr 2009

Keywords

  • Magnetic nanoparticles
  • MALDI MS
  • Microwave-heating
  • Niobium oxide
  • Phosphopeptides

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