Investigation of two-photon excited fluorescence increment via crosslinked bovine serum albumin

Chun Yu Lin*, Chi Hsiang Lien, Keng Chi Cho, Chia Yuan Chang, Nan Shan Chang, Paul J. Campagnola, Chen Yuan Dong, Shean Jen Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


The two-photon excited fluorescence (TPEF) increments of two dyes via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking technique were investigated. One is Rose Bengal (RB) with a high non-radiative decay rate, while the other is Eosin Y with a low non-radiative decay rate. Experimental results demonstrate that the quantum yield and lifetime of RB are both augmented via crosslinked BSA microstructures. Compared with theoretical analysis, this result indicates that the non-radiative decay rate of RB is decreased; hence, the quenched effect induced by BSA solution is suppressed. However, the fluorescence lifetime of Eosin Y is acutely abated despite the augmented quantum yield for the two-photon crosslinking processing from BSA solution. This result deduces that the radiative decay rate increased. Furthermore, the increased TPEF intensity and lifetime of RB correlated with the concentration of fabricated crosslinked BSA microstructures through pulse selection of the employed femtosecond laser is demonstrated and capable of developing a zone-plate-like BSA microstructure.

Original languageEnglish
Pages (from-to)13669-13676
Number of pages8
JournalOptics Express
Issue number13
StatePublished - 18 Jun 2012

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