Intravital imaging of ischemia and reperfusion

Ian Liau*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Timely evaluation of specific pathogenic mechanisms that underlie ischemia-reperfusion injury is essential to improve our understanding of the pathogenesis and to help develop effective therapies. Herein we report employment of intravital microscopy to visualize ischemia and reperfusion of rat liver in real time in vivo. We show that the time-lapse autofluorescence images exhibited a unique spatiotemporal change during the time course of ischemia and reperfusion. The intensity decreased rapidly after ischemia, but recovered gradually during reperfusion, with a rate depending strongly on the duration of ischemia. The intravital imaging further enabled direct visualization of occlusion of local microcirculation and showed that it led to delayed reoxygenation of cells, a pathogenic mechanism that would inevitably contribute tissue damage. Supported with complimentary hypoxia-reoxygenation experiments and inhibitory assays performed on cultured hepatocytes, we conclude that the variation of autofluorescence is mechanistically linked with the conversion of mitochondrial flavoproteins between the non-fluorescent reduced state and fluorescent oxidized state.

Original languageEnglish
Title of host publication2013 Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2013
DOIs
StatePublished - 18 Oct 2013
Event10th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2013 - Kyoto, Japan
Duration: 30 Jun 20134 Jul 2013

Publication series

NamePacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest

Conference

Conference10th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2013
CountryJapan
CityKyoto
Period30/06/134/07/13

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