Interpretation on band-broadening in chromatography with spatial peak profiles obtained using whole-column detection

Shu Hui Lin, Tiing Yu*, Su Cheng Pai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Fifteen liquid chromatographic experiments were investigated using a whole-column detection (WCD) system and a conventional post-column UV/Vis detector. The peak widths obtained from chromatograms were found dependent on the retention factor; the larger the retention factor was the greater the peak width. However, the on-column spatial peak widths were dependent on the locations where they were measured in the column. The peak widths monitored at 17 cm from the column inlet were found essentially the same no matter what their retention factors were. In addition, a linear relationship was found etween the chromatographic peak width and the reciprocal of the average linear rate of the solute migration. The peak widths on chromatograms did not reflect how they appeared in the column; instead, the widths were determined by the solute speed passing the detector.

Original languageEnglish
Pages (from-to)480-484
Number of pages5
JournalJournal of the Chinese Chemical Society
Volume56
Issue number3
DOIs
StatePublished - 1 Jan 2009

Keywords

  • Chromatography
  • Liquid chromatography
  • Peak broadening
  • Whole-column detection

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