Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging

Yunzhao Wu, Yuqi Zhou, Chun Jung Huang, Hirofumi Kobayashi, Sheng Yan, Yasuyuki Ozeki, Yingli Wu, Chia Wei Sun, Atsushi Yasumoto, Yutaka Yatomi, Cheng Lei*, Keisuke Goda

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning.

Original languageEnglish
Pages (from-to)519-532
Number of pages14
JournalOptics Express
Volume28
Issue number1
DOIs
StatePublished - 6 Jan 2020

Fingerprint Dive into the research topics of 'Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging'. Together they form a unique fingerprint.

Cite this