Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging

Yunzhao Wu, Yuqi Zhou, Chun Jung Huang, Hirofumi Kobayashi, Sheng Yan, Yasuyuki Ozeki, Yingli Wu, Chia Wei Sun, Atsushi Yasumoto, Yutaka Yatomi, Cheng Lei*, Keisuke Goda

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning.

Original languageEnglish
Pages (from-to)519-532
Number of pages14
JournalOptics Express
Volume28
Issue number1
DOIs
StatePublished - 6 Jan 2020

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    Wu, Y., Zhou, Y., Huang, C. J., Kobayashi, H., Yan, S., Ozeki, Y., Wu, Y., Sun, C. W., Yasumoto, A., Yatomi, Y., Lei, C., & Goda, K. (2020). Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging. Optics Express, 28(1), 519-532. https://doi.org/10.1364/OE.380679