Integrin-mediated membrane blebbing is dependent on sodium-proton exchanger 1 and sodium-calcium exchanger, 1 activity

Yung Hsiang Yi, Yu Sun Chang, Chi Hung Lin, Tien Shen Lew, Chih Yung Tang, Wei Lien Tseng, Ching Ping Tseng, Szecheng J. Lo*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Integrin signaling and membrane blebbing modulate cell adhesion, spreading, and migration. However, the relationship between integrin signaling and membrane blebbing is unclear. Here, we show that an integrin-ligand interaction induces both membrane blebbing and changes in membrane permeability. Sodium-proton exchanger 1 (NHE1) and sodium-calcium exchanger 1 (NCX1) are membrane proteins located on the bleb membrane. Inhibition of NHE1 disrupts membrane blebbing and decreases changes in membrane permeability. However, inhibition of NCX1 enhances cell blebbing; cells become swollen because of NHE1 induced intracellular sodium accumulation. Our study found that NHE1 induced sodium influx is a driving force for membrane bleb growth, while sodium efflux (and calcium influx) induced by NCX1 in a reverse mode results in membrane bleb retraction. Together, these findings reveal a novel function for NHE1 and NCX1 in membrane blebbing and permeability, and establish a link between membrane blebbing and integrin signaling.

Original languageEnglish
Pages (from-to)10316-10324
Number of pages9
JournalJournal of Biological Chemistry
Volume287
Issue number13
DOIs
StatePublished - 23 Mar 2012

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