Abstract
Histopathological imaging of tissues often requires extensive sample preparation including fixation and staining in order to highlight characteristic alterations associated with diseases. Herein, we report an integrated spectro-microscopy approach based on a combination of multi-modal multi-photon imaging and Raman micro-spectroscopy and demonstrate label-free characterization of the structure-constituent correlation of porcine skin. The multi-modal imaging allows the visualization of dermatological features whereas Raman micro-spectroscopy enables the identification of their 'molecular fingerprints'. By obtaining both structural and molecular-level information of tissue constituents, this integrated approach can offer new insight into the patho-physiological status of tissues.
Original language | English |
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Pages (from-to) | 16431-16441 |
Number of pages | 11 |
Journal | Optics Express |
Volume | 16 |
Issue number | 21 |
DOIs | |
State | Published - 13 Oct 2008 |