Integrated multiple multi-photon imaging and Raman spectroscopy for characterizing structure-constituent correlation of tissues

Jhen Wei Jhan, Wei Tien Chang, Hung Che Chen, Yu Tsung Lee, Ming Fang Wu, Chung Hsuan Chen, Ian Liau*

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Histopathological imaging of tissues often requires extensive sample preparation including fixation and staining in order to highlight characteristic alterations associated with diseases. Herein, we report an integrated spectro-microscopy approach based on a combination of multi-modal multi-photon imaging and Raman micro-spectroscopy and demonstrate label-free characterization of the structure-constituent correlation of porcine skin. The multi-modal imaging allows the visualization of dermatological features whereas Raman micro-spectroscopy enables the identification of their 'molecular fingerprints'. By obtaining both structural and molecular-level information of tissue constituents, this integrated approach can offer new insight into the patho-physiological status of tissues.

Original languageEnglish
Pages (from-to)16431-16441
Number of pages11
JournalOptics Express
Volume16
Issue number21
DOIs
StatePublished - 13 Oct 2008

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