Inhibitory effects of chloroform extracts derived from corbicula fluminea on the release of pro-inflammatory cytokines

Ching Min Lin, Yu Ling Lin, Nu Man Tsai, Hsin Yi Wu, Shu Yi Ho, Chia Hung Chen, Yen Ku Liu, Yi Han Chiu, Li Ping Ho, Ru Ping Lee*, Kuang-Wen Liao

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Corbicula fluminea, the primary freshwater bivalve cultivated in Taiwan, was formerly used as a remedy for hepatitis. Recent reports indicate that C. fluminea has many bioactivities, but it remains unknown whether C. fluminea affects inflammation. This study explored the anti-inflammatory activity of C. fluminea. C. fluminea was first treated with chloroform to obtain clam chloroform extracts (CCEs). On the basis of the assay for the release of pro-inflammatory cytokines in vitro and in vivo, the results show that the CCEs significantly lowered the release of lipopolysaccharide (LPS)-induced pro-inflammatory cytokines. Additionally, the CCEs reduced LPS-induced organ damage. Real-time polymerase chain reaction analysis suggested that CCEs inhibit the LPS-induced mRNA expression of interleukin-1β and tumor necrosis factor-α. Western blot analysis indicated that the CCEs increased expression of IκB and attenuated the phosphorylation of IκB. Gas chromatography-mass spectrometry suggests that phytosterols and fatty acids are responsible for the anti-inflammatory properties of CCEs. Taken together, CCEs have the potential to be developed as an anti-inflammatory functional food.

Original languageEnglish
Pages (from-to)4076-4082
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number16
DOIs
StatePublished - 25 Apr 2012

Keywords

  • Freshwater clam
  • IκB-α
  • IL-1β
  • inflammation
  • TNF-α

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    Lin, C. M., Lin, Y. L., Tsai, N. M., Wu, H. Y., Ho, S. Y., Chen, C. H., Liu, Y. K., Chiu, Y. H., Ho, L. P., Lee, R. P., & Liao, K-W. (2012). Inhibitory effects of chloroform extracts derived from corbicula fluminea on the release of pro-inflammatory cytokines. Journal of Agricultural and Food Chemistry, 60(16), 4076-4082. https://doi.org/10.1021/jf2051202