Inhibitory Effect of Excessive Glucose on Its Biochemical Pathway and the Growth of Chinese Hamster Ovary (CHO) Cells

Tsung Yih Lee, Hsiao Hsien Lin, Chien Lung Chen, Shiaw Min Hwang, Ching-Ping Tseng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In this study, we found the specific growth rate of CHO cells being reduced when glucose increased from 1 to 10 g/L. Under high glucose, we show a 41% increase in lactate dehydrogenase activity consistent with a 70% rate increase in lactate production. The pyruvate kinase activity was significantly increased. However, the activities of citrate synthase, isocitrate dehydrogenase, and malate dehydrogenase involved in the TCA cycle were attenuated. We propose that pyruvate produced from glycolysis is utilized in a lesser extent over the TCA cycle under high-glucose conditions. Thus, the pyruvate is largely converted to lactate, which is toxic to the cells.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalJournal of Carbohydrate Chemistry
Volume34
Issue number1
DOIs
StatePublished - 2 Jan 2015

Keywords

  • CHO cells
  • Glucose and TCA cycle enzymes
  • Glucose flux in glycolysis
  • Growth rate
  • ICDH

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