Inhibition of the lethality of Shiga-like toxin-1 by functional gold nanoparticles

Chun Hsien Li, Yi Ling Bai, Yu-Chie Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Escherichia coli O157:H7 is a pathogen, which can generate Shiga-like toxins (SLTs) and cause hemolytic-uremic syndrome. Foodborne illness outbreaks caused by E. coli O157:H7 have become a global issue. Since SLTs are quite toxic, effective medicines that can reduce the damage caused by SLTs should be explored. SLTs consist of a single A and five B subunits, which can inhibit ribosome activity for protein synthesis and bind with the cell membrane of host cells, respectively. Pigeon ovalbumin (POA), i.e. a glycoprotein, is abundant in pigeon egg white (PEW) proteins. The structure of POA contains Gal-α(1→4)-Gal-β(1→4)-GlcNAc ligands, which have binding affinity toward the B subunit in SLT type-1 (SLT-1B). POA immobilized gold nanoparticles (POA-Au NPs) can be generated by reacting PEW proteins with aqueous tetrachloroauric acid in one-pot. The generated POA-Au NPs have been demonstrated to have selective trapping-capacity toward SLT-1B previously. Herein, we explore that POA-Au NPs can be used as protective agents to neutralize the toxicity of SLT-1 in SLT-1-infected model cells. The results show that the cells can be completely rescued when a sufficient amount of POA-Au NPs is used to treat the SLT-1-infected cells within 1 h.

Original languageEnglish
Pages (from-to)841-851
Number of pages11
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume46
Issue numbersup1
DOIs
StatePublished - 31 Oct 2018

Keywords

  • Escherichia coli O157:H7
  • Shiga like toxin-1
  • artificial cells
  • gold nanoparticles
  • microcapsules
  • pigeon egg whites
  • pigeon ovalbumin
  • targeted delivery

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