Inhibition of hTAF(II)32-binding implicated in the transcriptional repression by central regions of mutant p53 proteins

Shiu Feng Tung, Jing Yuan Chuang, Chin Tarng Lin, Ming Yang Lai, Cheng-Wen Wu Lee, Young Sun Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We previously identified a movable and regulable inactivation function within the central region (CR(ts247)) of a temperature-sensitive p53 (p53(ts)) mutant, p53(N2471). Here we showed that central regions from several p53(ts) mutants behaved similarly, i.e. they repressed a neighboring activation domain only when existing in the mutant status. Using chimeric protein GAL4VP16-CR(ts247) as an example, we demonstrated that de novo protein synthesis was not required for the reactivation of the chimeric protein, indicating that a post-translational mechanism was involved in the control of CR(ts247) activity. The CR(ts247)-conferred thermo-regulability did not work via a mechanism demanding either an alteration of the subcellular compartmentalization of or the inactivation of DNA-binding activity of the GAL4 chimera. Further, CR(ts247) did not function in trans, eliminating the possibility that the observed repression was because of the competition for a putative factor(s) by the mutant p53 domain. Rather, CR(ts247) bestowed temperature-dependent interaction with hTAF(II)32 to the VP16 activation domain. In a parallel experiment, CR(ts247) also caused a large reduction in the affinity of hTAF(II)32 to the p53 activation domain at the nonpermissive temperature. These results strongly suggested that inhibition of hTAF(II)32 binding could be one of the mechanisms responsible for the transcriptional repression by mutant p53 central regions.

Original languageEnglish
Pages (from-to)7748-7755
Number of pages8
JournalJournal of Biological Chemistry
Volume274
Issue number12
DOIs
StatePublished - 19 Mar 1999

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